ldn-193189 (MedChemExpress)
Structured Review
Ldn 193189, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ldn+193189/LDN-193189/custom%40hy-12071b%4042421304
Average 94 stars, based on 11 article reviews
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Cell Culture:Article Title: Quantifying the Single‐Cell Morphological Landscape of Cellular Transdifferentiation through Force Field Reconstruction Article Snippet: .. Cells were then washed three times with PBS and cultured in fibroblasts medium for 24 h. Then medium was changed to neural induction medium (NDiff227; Takara‐Clontech) supplemented with growth factors at the following concentrations: LM‐22A4 (2 × 10 −6 m , Tocris), GDNF (2 ng mL −1 , R&D Systems), NT3 (10 ng μL −1 , R&D Systems) and db‐cAMP (0.5 × 10 −3 m , Stemcell), and the small molecules CHIR99021 (2 × 10 −6 m , MedChemExpress), SB‐431542 (10 × 10 −6 m , MedChemExpress), noggin (0.5 μg mL −1 , R&D Systems), Suspension:Article Title: Construction of a Duck Intestinal Organoid Culture System: From Crypt Isolation to Medium Optimization. Article Snippet: .. After the crypt suspension was obtained, the following three types of culture media were used for cultivation: (1) ENR medium: This medium contained 1 mg/mL epidermal growth factor (EGF, HY-P7109, MedChemExpress), 100 μg/mL Noggin (HYP700143AF, MedChemExpress), and 500 μg/mL R-spondin 1 (HY-P7114, MedChemExpress). (2) Organoid growth medium (OGM): This was a commercial medium (06005, STEMCELL Technologies Inc., Vancouver, BC, Canada). (3) CL medium: This medium contained 15 μM CHIR-99021 (HY-10182, MedChemExpress) and 0.3 μM In Vitro:Article Title: Vitamin D supplementation ameliorates anemia of inflammation by reducing hepcidin levels and inactivating inflammatory signaling pathways. Article Snippet: The hepatoblastoma cell line HepG2 (Procell, Wuhan, China) was cultured in Dulbecco’s modified Eagle medium (DMEM; Procell) supplemented with 10% fetal bovine serum (FBS; Procell) and 1% penicillin/streptomycin (Beyotime, Shanghai, China) in a humidified incubator with 5% CO2 at 37 °C. .. To simulate inflammation in vitro, HepG2 cells were stimulated with 1 μg/mL LPS (Beyotime) for 24 h. To examine vitamin D’s role in LPS-induced inflammation, HepG2 cells were exposed to different concentrations (15, 25, and 50 ng/mL) of the active form of vitamin D (1,25(OH)2D3; MedChemExpress, Shanghai, China) in the presence of LPS for 24 h. To elucidate the involvement of signaling pathways, HepG2 cells were treated with 10 μM BAY11-7082 (NF-κB inhibitor), 25 μM AG490 (JAK2 inhibitor), or 5 μM Protein-Protein interactions:Article Title: Vitamin D supplementation ameliorates anemia of inflammation by reducing hepcidin levels and inactivating inflammatory signaling pathways. Article Snippet: The hepatoblastoma cell line HepG2 (Procell, Wuhan, China) was cultured in Dulbecco’s modified Eagle medium (DMEM; Procell) supplemented with 10% fetal bovine serum (FBS; Procell) and 1% penicillin/streptomycin (Beyotime, Shanghai, China) in a humidified incubator with 5% CO2 at 37 °C. .. To simulate inflammation in vitro, HepG2 cells were stimulated with 1 μg/mL LPS (Beyotime) for 24 h. To examine vitamin D’s role in LPS-induced inflammation, HepG2 cells were exposed to different concentrations (15, 25, and 50 ng/mL) of the active form of vitamin D (1,25(OH)2D3; MedChemExpress, Shanghai, China) in the presence of LPS for 24 h. To elucidate the involvement of signaling pathways, HepG2 cells were treated with 10 μM BAY11-7082 (NF-κB inhibitor), 25 μM AG490 (JAK2 inhibitor), or 5 μM other:Article Title: Bone morphogenetic protein-2 and transforming growth factor-β1 regulate cell proliferation and ectopic claudin-4 expression in parotid acinar cells Article Snippet: |
